scholarly journals Lysophosphatidic acid, a novel lipid growth factor for human thyroid cells: Over-expression of the high-affinity receptor edg4 in differentiated thyroid cancer

2001 ◽  
Vol 92 (2) ◽  
pp. 249-256 ◽  
Author(s):  
Klaus-Martin Schulte ◽  
Andreas Beyer ◽  
Karl K�hrer ◽  
Simone Oberh�user ◽  
Hans-Dietrich R�her
Keyword(s):  
Thyroid Cancer ◽  
Growth Factor ◽  
Lysophosphatidic Acid ◽  
Differentiated Thyroid Cancer ◽  
Human Thyroid ◽  
Thyroid Cells ◽  
Over Expression ◽  
High Affinity ◽  
High Affinity Receptor ◽  
Affinity Receptor

Presence of epidermal growth factor receptors on human thyroid membranes

1987 ◽  
Vol 114 (3) ◽  
pp. 396-401 ◽  
Author(s):  
Kikuo Kasai ◽  
Masaki Hiraiwa ◽  
Yoshinobu Suzuki ◽  
Tatsushi Emoto ◽  
Nobuyuki Banba ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Human Thyroid ◽  
Tissue Homogenate ◽  
Dependent Manner ◽  
Egf Receptors ◽  
Thyroid Cells ◽  
High Affinity Receptor ◽  
Epidermal Growth ◽  
Affinity Receptor

Abstract. The nature of epidermal growth factor (EGF) receptors in normal and pathological thyroid membranes was studied on a crude membrane fraction (10 000 × g pellet) of tissue homogenate. Optimal binding of 125I-EGF to human thyroid membranes was obtained at 25°C with 1-h incubation at pH 7.4. The study revealed the presence of both high and low affinity receptors in normal and pathological thyroid membranes. The association constants of high affinity receptor (3.2 × 10−9 mol/l) and of low affinity receptor (1.8 × 10−8 mol/l) observed in normal thyroid membranes were similar to those of thyroid membranes from neoplastic as well as thyrotoxic thyroid tissues. [3H]thymidine incorporation into DNA of cultured human thyroid cells was stimulated by EGF in a dose-dependent manner. A half-maximal stimulation was found around 1 × 10−10 mol/l. These results suggest that EGF may have a possible role in the regulation of thyroid growth and function in physiological and pathological situations.

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